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. 2022 Nov 7;4(11):1532–1550. doi: 10.1038/s42255-022-00672-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a, Time spent feeding on 1 or 10% sucrose using FLIC; all n = 12 animals. b,c, Preference between 1 versus 10% sugar measured over 6 or 24 hours by CAFÉ assay. b, 6-hour preference: n = 10 EEC>, n = 9 EEC>NPFi^KK. 24-hour preference: n = 10 each. c, n = 14 NPF^gut>, n = 15 NPF^gut>NPFi^TRiP. d, Midgut NPF staining intensity in fed females on a per-cell basis and on a per-gut basis; n = 703 cells from seven guts for virgins, n = 883 cells from seven guts from mated females. e, NPF-cell activity measured in dissected midguts (two midguts per replicate) using a luciferase-based CaLexA calcium-reporter system (NPF>LexA::NFAT::VP16; LexAop-luciferase); n = 4 from fed virgins, n = 4 from fed females mated to SP-deficient males (SP⁰/Df(3L)delta130), n = 6 from fed females mated to SP⁺ males. f, Consumption preference for 10% sucrose versus 10% yeast after 3 days of yeast deprivation (3 days on sucrose-only medium) using two-choice dye assay; n = 25 NPF^gut> virgins, n = 23 NPF^gut>NPFi^TRiP virgins, n = 24 NPF^gut> mated females, n = 22 NPF^gut>NPFi^TRiP mated females, n = 17 EEC> virgins, n = 18 EEC>NPFi^sh virgins, n = 18 EEC> mated females, n = 18 EEC>NPFi^sh mated females. g, Yeast consumption determined by dye assay; virgins n = 10 NPF^gut> virgins, n = 6 NPF^gut>NPFi^TRiP virgins, n = 9 NPF^gut> mated females, n = 8 NPF^gut>NPFi^TRiP mated females. h, Yeast intake determined by CAFÉ; n = 32 NPF^gut>, n = 26 NPF^gut>NPFi^TRiP. i, Cumulative behavioural preference of females for sucrose versus yeast monitored using flyPAD. Lines represent means, and shading indicates s.e.m.; n = 24 NPF^gut> virgins, n = 23 NPF^gut>NPFi^TRiP virgins, n = 24 NPF^gut> mated females, n = 24 NPF^gut>NPFi^TRiP mated females. Bars represent mean ± s.e.m. Box plots indicate minimum, 25th percentile, median, 75th percentile and maximum values. NS, not significant. a,c,d (left), Two-tailed unpaired Mann–Whitney U-test. b,d (right), h, Two-tailed unpaired Student’s t-test. e,f (right), g, One-way ANOVA with Tukey’s post hoc test. f (left), i, Kruskal–Wallis nonparametric ANOVA with Dunn’s multiple-comparisons test.

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