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. 2022 Nov 7;4(11):1532–1550. doi: 10.1038/s42255-022-00672-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, Intake of sucrose or yeast measured by dye assay in females mated to SP-producing or SP-deficient males (SP⁰/Df(3L)delta130). Sucrose, n = 15 NPF^gut> females mated to SP⁺ males, n = 15 NPF^gut> females mated to SP-mutant (SP^null) males, n = 14 NPF^gut>NPFi^TRiP females mated to SP⁺ males. Yeast, n = 15 NPF^gut> females mated to SP⁺ males, n = 20 NPF^gut> females mated to SP-mutant males, n = 13 NPF^gut>NPFi^TRiP females mated to SP⁺ males. b, Consumption preference using two-choice dye assay; n = 25 NPF^gut> virgins, n = 24 NPF^gut> females mated to SP⁺ males, n = 18 NPF^gut> females mated to SP-null males, n = 21 NPF^gut>NPFi^TRiP females mated to SP⁺ males. c,d, Consumption preference of w¹¹¹⁸ virgin females with or without NPF injection (c) and virgin and mated females (d) (with and without NPF injections) with knockdown of SPR in Ppk⁺ neurons (ppk>SPRi) using two-choice dye assay. c, n = 19 w¹¹¹⁸ virgins, n = 20 w¹¹¹⁸ virgins with NPF injection. d, n = 11 virgin ppk>SPRi females, n = 12 mated ppk>SPRi females, n = 12 mated ppk>SPRi females with NPF injection. Bars represent mean ± s.e.m. Box plots indicate minimum, 25th percentile, median, 75th percentile and maximum values. NS, not significant. a (left), b,d, One-way Kruskal–Wallis ANOVA with Dunn’s multiple-comparisons test. a, Right, one-way ANOVA with Dunnett’s multiple-comparisons test. c, Two-tailed unpaired Mann–Whitney U-test.

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