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. 2022 Nov 22;13:7160. doi: 10.1038/s41467-022-34702-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Northern blot of DIO3OS-knockdown efficiency in MCF-7/T47D LTED cells by the indicated LNAs. ACTB serves as loading control RNA. nt, nucleotide. b Growth curves of MCF-7/T47D LTED cells transfected with control or DIO3OS-targeting LNAs. c Representative immunofluorescence images and quantification of EdU-incorporated MCF-7 LTED cells transfected with control or DIO3OS-targeting LNAs. Scale bars, 50 μm. d Representative images and quantification of plate clone formation of MCF-7/T47D LTED cells transfected with control or DIO3OS-targeting LNAs. e, f ECAR values and calculated glycolytic capacity of MCF-7 LTED (e) and T47D LTED (f) transfected with control or DIO3OS-targeting LNAs. g Lactate production of MCF-7/T47D LTED cells transfected with control or DIO3OS-targeting LNAs. h Northern blot of DIO3OS overexpression efficiency in parental MCF-7/T47D cells. ACTB serves as loading control RNA. nt, nucleotide. i Growth curves of parental MCF-7/T47D cells transfected with control or DIO3OS overexpression plasmids. j Representative immunofluorescence images and quantification of EdU-incorporated parental MCF-7 cells transfected with control or DIO3OS overexpression plasmids. Scale bars, 50 μm. k Representative images and quantification of plate clone formation of parental MCF-7/T47D cells transfected with control or DIO3OS overexpression plasmids. l Lactate production of parental MCF-7/T47D cells transfected with control or DIO3OS overexpression plasmids. m, n ECAR values and calculated glycolytic capacity of parental MCF-7 (m) and T47D (n) cells transfected with control or DIO3OS overexpression plasmids. Means ± s.d. of experimental triplicates (b–g, i–n), one representative experiment out of three that were similar (a, h) are shown, and P-values were assessed with two-sided one-way ANOVA with Dunnett’s multiple-comparisons test. Source data are provided as a Source Data file.