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. 2022 Nov 22;13:7160. doi: 10.1038/s41467-022-34702-x

Fig. 4. DIO3OS regulates LTED cell proliferation and glycolysis through PTBP1.

Fig. 4

a Lactate production of MCF-7/T47D LTED cells co-transfected with control LNA/DIO3OS-targeting LNA and control vector/PTBP1 overexpression plasmid. b, c ECAR values and calculated glycolytic capacity of MCF-7 LTED (b) and T47D LTED (c) cells with the indicated treatments. d Lactate production of parental MCF-7/T47D cells transfected with control construct or co-transfected with DIO3OS expression plasmid and PTBP1 siRNAs or control siRNA. e, f ECAR values and calculated glycolytic capacity of parental MCF-7 (e) and T47D (f) cells with the indicated treatments. g–i Cell number counting of MCF-7/T47D LTED cells (g) and parental MCF-7/T47D cells (h, i) with the indicated treatments. j Representative immunofluorescence images and quantification of EdU-incorporated MCF-7 LTED cells with the indicated treatments. Scale bars, 50 μm. k Representative images and quantification of plate clone formation of MCF-7 LTED cells with the indicated treatments. Means ± s.d. of experimental triplicates (a–k) are shown, and all P-values were analyzed using two-sided one-way ANOVA with Dunnett’s multiple-comparisons test. Source data are provided as a Source Data file.