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. 2022 Nov 22;13:7152. doi: 10.1038/s41467-022-34894-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — The protein kindlin-GFP was visualized using a DNA-labeled GFP nanobody and imaged via DNA-PAINT. a 5 × 5 μm² region of traditional super-resolution image. b BaGoL posterior image of panel a. c BaGoL MAPN image of panel a. d Zoom-in of the green box in panel a. Blue circles represent the found emitter coordinates by BaGoL. e Zoom-in of the green box in panel c. f Histograms of the localization precisions from SR data shown in brown (input to BaGoL), and the improved precisions from BaGoL shown in blue. BaGoL was applied to the focal adhesion protein kindlin data for three similar regions. Scale bars in the top and bottom rows are 1000 and 100 nm, respectively.