Skip to main content
. 2022 Nov 10;9:989670. doi: 10.3389/fvets.2022.989670

Figure 3.

Figure 3

Primary culture of postmortem fishing cats. (A–C) Primary culture of tissues collected from ear pinna of postmortem fishing cats. (A,B) young fishing cat at age 2 months. (C) Adult fishing cat at age 12 years. No cells expanded from ear pinna explant in primary culture at day 30. (D–J) Primary culture of tissues collected from testis of postmortem fishing cat (adult at age 12 years). (D) Male reproductive organ of fishing cat collected for primary culture. (E) Fibroblast outgrowth from tunica albuginea explants. (F) Fibroblast outgrowth from epididymis explants. (G,H) Fibroblasts from the testes at passage 1 and passage 4 were tested for cell senescence. (I) Live alkaline phosphatase (Live-AP) staining was used to detect the presence of putative spermatogonial stem cells (SSCs) from seminiferous tubules and epididymis. Green color indicates possible signal for AP activity. (J) Immunostaining of seminiferous tubule extract to detect SOX2 protein (Red, Alexa647) with nuclear staining Hoechst33342. Inset shows zoom-up of SOX2-positive cells.