Table 2.
Antibodies used for immunofluorescence and Western Blot detection
| Antibody name | Method | Antibody typ | Source | Catalogue nr | Dilution |
|---|---|---|---|---|---|
| anti-PLIN1 (anti-PLIN1 rabbit polyclonal antibody) | IF | primary |
Abcam Cambridge, UK |
ab3526 RRID:AB_2167274 |
1:10 000 |
| anti-PLIN2 (ADRP (Perilipin 2) (AA5-27 AP125) mouse monoclonal antibody) | IF | primary |
AntibodiesOnline Aachen, Germany |
ABIN112185 RRID:AB_2915956 |
1:500 |
| anti-PLIN3 (TIP47 (F-10) mouse monoclonal antibody) | IF | primary |
Santa Cruz Biotechnologies Santa Cruz, CA, USA |
sc-390968 RRID:AB_2915955 |
1:5 000 |
| BrightVision, 1 step detection system goat anti- mouse HRP | IF | secondary |
ImmunoLogic Duiven, Netherlands |
DPVM110HRP RRID:AB_2915957 |
RTU |
| BrightVision, 1 step detection system goat anti- rabbit HRP | IF | secondary |
ImmunoLogic Duiven, Netherlands |
DPVR110HRP RRID:AB_2915958 |
RTU |
| anti-PLIN1 (anti-PLIN1 rabbit polyclonal antibody) | WB | primary |
Abcam Cambridge, UK |
ab3526 RRID:AB_2167274 |
1:500 |
| anti-PLIN2 (ADRP/Perilipin 2 rabbit polyclonal antibody) | WB | primary |
Proteintech Rosemont, IL, USA |
15,294–1-AP RRID:AB_2878122 |
1:500 |
| anti-PLIN3 (TIP47 (F-10) mouse monoclonal antibody) | WB | primary |
Santa Cruz Biotechnologies Santa Cruz, CA, USA |
sc-390968 RRID:AB_2915955 |
1:500 |
| anti-GAPDH (GAPDH [GT239] mouse monoclonal antibody) | WB | primary |
GeneTex Irvine, CA, USA |
GTX627408 RRID:AB_11174761 |
1:5 000 |
| anti-α-tubulin (anti-α-tubulin rabbit polyclonal antibody) | WB | primary |
Abcam Cambridge, UK |
ab4074 RRID:AB_2288001 |
1:1 000 |
| Amersham ECL Rabbit IgG, HRP-linked whole Ab (donkey) | WB | secondary |
GE Healthcare Chicago, IL, USA |
NA934 RRID:AB_772206 |
1:5 000 |
| Amersham ECL Mouse IgG, HRP-linked whole Ab (sheep) | WB | secondary |
GE Healthcare Chicago, IL, USA |
NA931 RRID:AB_772210 |
1:5 000 |
IF – immunofluorescence; WB – Western Blot; RTU – ready to use