Table 3.
Primer for RT-qPCR
| Gene symbol | Gene name | NCBI accession number | Oligo sequence (5 ‘—3 ‘) | Amplicon length (bp) | PCR efficiency (%) | R2 value | Reference |
|---|---|---|---|---|---|---|---|
| PLIN1 | Perilipin 1 | XM_038661340.1 |
F: GTACCCTCCTGAGAAGATTG R: GGGCACACTGATGCTATT |
85 | 98 | 0.993 | - |
| PLIN2 | Perilipin 2 | XM_005626663.3, XM_003639380.4 |
F: AATTTGCCAGAAAGAATGTGCAT R: TCCACCCAGGAGAGGTAGAACTT |
79 | 99 | 0.991 | - |
| PLIN3 | Perilipin 3 | XM_038429023.1, XM_038429022.1 |
F: GGGTCAGGAGAAACTACAC R: GTCTCCACCTCTGGTTTG |
93 | 97 | 0.995 | - |
| OAZ1 | Ornithine decarboxylase antizyme 1 | NM_001127234.1 |
F: CTGCTGTAGTAACCTGGGTC R: ACATTCAGCCGATTATCAGAGTA |
145 | 97 | 0.994 | Gabriel et al. 2016 |
| RPL27 | Ribosomal protein L27 | NM_001003102.2 |
F: ACTACAATCACCTCATGCCC R: CTTGTACCTCTCCTCGAACTTG |
143 | 94 | 0.998 | Gabriel et al. 2016 |
| RPL32 | Ribosomal protein L32 | NM_001252169.1 |
F: TGGCCATCAGAGTCACCAATC R: GACGCGCACATAAGCTGTTTAT |
74 | 94 | 0.998 | - |
| RPL8 | Ribosomal protein L8 | XM_853403.4 |
F: TCTTCCGCCAACAGAGCC R: CTTTGCCTTGTACTTGTGGTAAGC |
102 | 94 | 0.995 | - |
R2: correlation coefficient of standard curve; F and R: forward and reverse primer