TABLE 3.
Influence of adjuvants on the immune response towards LPS-deficient OMCs
| Adjuvant | Dose (μg)a | ELISA titerb
|
Bactericidal titerc (H44/76) | ||||
|---|---|---|---|---|---|---|---|
| IgG | IgG1 | IgG2a | IgG2b | IgG3 | |||
| H44/76 LPS | 5 | 4.8 | 3.5 | 4.6 | 4.3 | 3.6 | 4.4 |
| icsB LPS | 5 | 4.7 | 3.5 | 4.4 | 4.3 | 3.2 | 3.9 |
| rfaC LPS | 5 | 4.9 | 3.6 | 4.8 | 4.6 | 3.1 | 4.1 |
| htrB1 LPS | 5 | 4.4 | 3.6 | 4.0 | 4.0 | 3.3 | 4.2 |
| E. coli LPS | 5 | 4.9 | 3.9 | 4.6 | 4.5 | 3.4 | 4.1 |
| Quil A | 20 | 4.6 | 3.7 | 4.6 | 4.2 | 3.4 | 3.9 |
| H44/76 dLPS | 5 | 4.2 | 3.4 | 3.6 | 3.8 | 3.2 | 3.1 |
| R. sphaeroides LPS | 5 | 3.8 | 3.4 | 3.4 | 3.5 | 2.7 | 2.6 |
| MPL | 10 | 4.3 | 3.5 | 3.9 | 4.3 | 3.4 | 2.9 |
| MPL | 20 | 4.3 | 3.8 | 3.8 | 4.2 | 3.8 | 3.4 |
| AlPO4 | 500 | 4.2 | 3.7 | 3.9 | 4.0 | 2.8 | 3.1 |
a
Mice were immunized with 20 μg of LPS− H44/76 OMCs supplemented with a dose of adjuvant as indicated.
b
The titer of each anti-H44/76 IgG isotype was determined by whole-cell ELISA and is expressed as the mean log10 titer. The means were compared by the LSD test with a confidence level of 95%: LSD0.05 (IgG) = 0.4; LSD0.05 (IgG1) = 0.3; LSD0.05 (IgG2a) = 0.4; LSD0.05 (IgG2b) = 0.5; LSD0.05 (IgG3) = 0.5.
c
The bactericidal titer was determined as the reciprocal value of the serum dilution which is effective for >90% killing of strain H44/76 and is expressed as the mean log10 titer. The means were compared by the LSD test with a confidence level of 95%: LSD0.05 = 0.9.