TABLE 4.
Adjuvant activity of Quil A fractions and MF59
| Immunogen | Dose (μg) | Adjuvant | ELISA titera
|
Bactericidal titerb (H44/76) | ||||
|---|---|---|---|---|---|---|---|---|
| IgG | IgG1 | IgG2a | IgG2b | IgG3 | ||||
| LPS− H44/76 OMC | 20 | None | 3.2 | 2.9 | 2.3 | 2.5 | 1.5 | 1.0 |
| LPS− H44/76 OMC | 20 | Quil A total | 4.6 | 3.8 | 4.7 | 3.9 | 3.3 | 3.3 |
| LPS− H44/76 OMC | 20 | QA3 | 4.5 | 3.7 | 4.3 | 3.9 | 2.4 | 3.8 |
| LPS− H44/76 OMC | 20 | QA17 | 5.0 | 3.8 | 4.8 | 4.3 | 3.0 | 3.5 |
| LPS− H44/76 OMC | 20 | QA20 | 4.7 | 3.3 | 5.0 | 3.9 | 3.3 | 3.6 |
| LPS− H44/76 OMC | 20 | QA22 | 4.5 | 4.0 | 4.4 | 3.8 | 2.4 | 4.0 |
| LPS− H44/76 OMC | 20 | QA23 | 4.4 | 3.9 | 4.2 | 3.5 | 2.4 | 3.9 |
| LPS− H44/76 OMC | 20 | MF59 | 4.0 | 4.0 | 3.5 | 3.3 | 2.5 | 3.3 |
a
The titer of each anti-H44/76 IgG isotype was determined by whole-cell ELISA and is expressed as the mean log10 titer. The means were compared by the LSD test with a confidence level of 95%: LSD0.05 (IgG) = 0.3; LSD0.05 (IgG1) = 0.4; LSD0.05 (IgG2a) = 0.5; LSD0.05 (IgG2b) = 0.4; LSD0.05 (IgG3) = 0.8.
b
The bactericidal titer was determined as the reciprocal value of the serum dilution which is effective for >90% killing of strain H44/76 and is expressed as the mean log10 titer. The means were compared by the LSD test with a confidence level of 95%: LSD0.05 = 1.2.