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. 2022 Nov 23;13:7181. doi: 10.1038/s41467-022-35000-2

Fig. 1. The asymmetrically localized PodJ is a self-assembled protein.

Fig. 1

a Schematic of asymmetric cell division of C. crescentus. The scaffolds PodJ-PopZ at the swarmer cell pole and PopZ-SpmX at the stalked cell pole recruit distinct signaling proteins, such as PleC and DivJ, respectively. After cell division, the swarmer cells develop into a stalked cell, which is correlated with the remodeling of the cell pole from a PodJ-rich signaling hub to an SpmX-rich signaling hub. b Detection of an intrinsically disordered region (IDR) in PodJ. The probability of IDR is represented as average scores (red line) calculated by Metadisorder MD259, SPOT60, Cspritz61, and IUPred262. Domain prediction was adapted from previous studies15, 19. TM transmembrane domain. c PodJ polar accumulation is independent of PopZ and TipN, but is affected by SpmX in C. crescentus. As a control, PleC accumulation was dependent on PodJ. A quantitative analysis of PodJ accumulation is shown on the right panel. d Heterologous expression of YFP-PodJ in E. coli indicates that PodJ accumulation is independent of polarity proteins. e Purified PodJ1-601 oligomerizes into a huge complex as illustrated by the native PAGE and compared with that of the SDS-PAGE analysis. Bovine serum albumin (BSA) was used as a control. M, protein marker. All scale bars, 1 μm. Source data are provided in the Source Data file.