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. 2022 Nov 23;13:7181. doi: 10.1038/s41467-022-35000-2

Fig. 6. Schematic of PodJ phase separation and the proposed model for regulating bacterial asymmetric cell division.

Fig. 6

a Functional domain architecture of PodJ. Both CC4–6 and IDR are responsible for PodJ phase separation in vitro. CC4–6 is the sole domain sufficient to drive PodJ LLPS in vivo and CC1–3 is the domain functioning in LLPS promotion. b The monomeric PodJ_N undergoes phase separation into biomolecular condensates, which are regulated by the concentrations of protein and salt in vitro. c Phase separation is involved in the regulation of PodJ new cell pole assembly and new-to-old cell-pole remodeling. The assembly of scaffold-signaling complexes at the new cell pole is mediated by PodJ phase separation. In addition, the subcellular accumulation and the client recruitment of PodJ are negatively regulated by SpmX, which is also through the LLPS of the two proteins.