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. 2022 Nov 23;13:7204. doi: 10.1038/s41467-022-34784-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Nuclear DNA base editing efficiencies of HEK293T cells treated with five 3ZF+3ZF nuclear-targeted ZF-DdCBE pairs, or ZF-DdCBE variants with extended ZF arrays. ZF-DdCBE pairs were designed to install edits within or nearby nuclear genes COL5A1, DCAF8L2, EMILIN2, EMILIN2, and TRAM1L1, respectively. The editing efficiencies shown are for the most efficiently edited C•G within the spacing region. b Nuclear DNA base editing efficiencies of HEK293T-HBB cells treated with an optimized ZF-DdCBE pair designed to correct the HBB −28A>G mutation. The DNA spacing region, split DddA orientation, ZF array lengths, and ZF-targeted DNA strands (LT left top, RB right bottom) are shown, and the pathogenic cytosine is colored in blue. For (a and b), values and errors reflect the mean ± s.d. of n = 3 independent biological replicates. Source data are provided as a Source Data file.