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. 2022 Nov 23;13:7200. doi: 10.1038/s41467-022-34679-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Western blot of lysates of frontal cortex obtained from nine brains (lanes 1–4, females; lanes 5–9, males) from control patients and nine brains (lanes 10–14, females; lanes 15–19, males) from AD patients. Western blots were probed with antibodies specific to total PKCα (top) and β-actin (bottom) as loading control. Graph depicts the quantification of the data in the western blot, including an additional 5 samples of each condition. b Western blot of lysates of frontal cortex obtained from five brains (lanes 1–2, males; lanes 3–5, females) from control patients and five brains (lanes 6–7, males; lanes 8–10, females) from AD patients. Western blots were probed with antibodies specific to a known PKC phosphorylation site on SAP97 (Thr656) (Top) or to total SAP97 (Bottom). Graph depicts the quantification of the data in the western blot. a, b Bands were quantified using densitometry and the phospho-SAP97 signal was normalized to total SAP97 signal and PKCα signal was normalized to its β-actin loading control. Normalized data from the depicted western blots were plotted (Bottom) as average normalized intensity ± SEM (p values were determined using unpaired two-tailed Student’s t test). Source data and uncropped blots for figures a and b are in the Source Data file.