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. 2022 Nov 23;12:20155. doi: 10.1038/s41598-022-24210-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Image processing. (a) Wide-field endoscopic image shows endomicroscope (E) placed in contact with normal (N) colonic mucosa for collection of fluorescence images in vivo following administration of fluorescein. (b) Drift in phase of the X and Y-axes during scanning may result in blurring from misregistered pixels. Large phase shifts were applied to the original image for demonstration. (c) After post-processed phase correction, mucosal details can be appreciated, including crypt structure (arrow) with central lumen (l) surrounded by lamina propria (lp). Individual cells can be distinguished, including colonocytes (c), goblet cells (g), and inflammatory cells (arrowhead). See Supplementary Video 1. (b, c) Images were processed using LabVIEW 2021.