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. 2022 Nov 23;12:20155. doi: 10.1038/s41598-022-24210-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Imaging system. (a) The PMT, laser, and amplifiers are contained within the base station. (b) In the filter block, the laser (blue) delivers excitation via a fiber pigtail via a FC/APC connector. The beam is deflected by a dichroic mirror (DM) into a single mode fiber (SMF) via a second FC/APC connector. Fluorescence (green) passes through the DM and a long pass filter (LPF) to the PMT via a multimode fiber (MMF). (c) The proximal end of the endomicroscope connects to the fiber (F) and wire (W) ports of the base station. (d) The endomicroscope, monitor, base station, computer, and isolation transformer are contained on a portable cart. (a, c) Solidworks 2016 was used for 3D modelling of the imaging system assembly and the endomicroscope.