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. 2022 Nov 23;13:7195. doi: 10.1038/s41467-022-34912-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — The T_m values of 70 single mutants (2–4 replicates) determined by using the Thermofluor assay are shown with dots and the average Tm values are shown in bars. The average T_m of wild-type EUGO is 66.5 °C and set as a reference. The seven most stabilizing mutations with a T_m increase of at least 2 °C that were studied in more detail are in dark orange bars. Two of the 72 FRESCO-predicted mutants could not be expressed and were not further studied.