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. 2022 Nov 10;16:994251. doi: 10.3389/fnins.2022.994251

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Copyright © 2022 Wu, Park, Kwon, Feng, Schweder, Dragunow, Shim and Rosset.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Brain pericytes isolated from human explants. (B) Dielectric elastomer actuators that produce uniaxial strain are fabricated. (C) Patient-matched brain pericyte cells are plated on our devices. (D) Our devices stretch the cultured pericyte cells 50 times at 20% strain. (E) Scratch assays were conducted using a custom-3D-printed scratch device as a preliminary step to determine which genes would respond to injury. (F) Polymerase chain reaction technique used to determine the genes that responds to injury from the scratch assays, the same genes were then investigated after injuring the pericytes with our stretch devices.