FIG. 5.

Nucleotide sequence of the IS1126 elements isolated in this study and the transposition site of IS1126₂. (A) Comparison of the nucleotide sequences and the deduced amino acid sequences of different IS1126 elements. Partial nucleotide and amino acid sequences of IS1126_Tn were compared with those of IS1126 from P. gingivalis W83 and IS1126₂. Boxes represent the 12-bp terminal inverted repeats of IS1126. The 5-bp nucleotide sequences flanking inverted repeats are duplicated target sequences generated after IS1126 transposition. Asterisks denote the 4-bp 5′-GAAG-3′ deletion found in IS1126_Tn which resulted in premature termination of IS1126 transposase synthesis. (B) Location of the duplicated copy of IS1126₂ found in P. gingivalis MSM-3 genome. A 4-kb BamHI restriction fragment (shaded) was cloned directly from MSM-3 chromosomal DNA into pGEM3Zf, and the nucleotide sequence was determined. Large arrows represent the size and orientation of the prtT, sod, prtC, and hmuR genes and of orfA. Small arrow in IS1126₂ indicates the direction of IS1126 transposase gene. prtT, cysteine protease gene; sod, superoxide dismutase gene; prtC, putative collagenase gene.