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. 2022 Nov 10;12:1042945. doi: 10.3389/fcimb.2022.1042945

Figure 4.

Figure 4

The detection of mNGS using cfDNA and wcDNA at different thresholds (RPM of 200, 100, 50, and 25). (A) Detection summary of fungi, viruses, and intracellular microbes by mNGS of both cfDNA and wcDNA at high and low loads. The number in the bubble above the horizontal axis is the ratio of the microbes with more RPM by mNGS of cfDNA than mNGS of wcDNA to the whole microbes at this threshold, while the number in the bubble below the horizontal axis is the ratio of the microbes with more RPM by mNGS of wcDNA than mNGS of cfDNA. Besides, vertical axis coordinate is the ratio of the numbers in the bubble. (B) Detection summary of fungi, viruses, and intracellular microbes only detected by mNGS of cfDNA or wcDNA at high and low loads. The column is the ratio of the microbes only detected by mNGS of cfDNA or wcDNA to the whole microbes at this threshold. The number above the column is the ratio of the microbes only detected by mNGS of cfDNA divided by that only detected by mNGS of wcDNA.