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FACE analysis of sugars from vaginal fluid samples. Lane 1, glucose polymer standard; lanes 2, 4, and 6, free oligosaccharides generated from three different samples (I, II, and III) by precipitation of vaginal fluid with ethanol. Total oligosaccharides (generated by treatment of vaginal fluid with N- and O-glycosidases) prior to ethanol precipitation from the same three samples are shown in the adjacent lanes (lanes 3, 5, and 7). See text for details.
