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Bacterial overlay assay using 35S-labeled type 1-piliated E. coli (HB101/pWRS1-17). Silver-stained gels of vaginal fluid before (A) and after (B) deglycosylation. The proteins albumin (alb) and transferrin (tf) are indicated. (C) Autoradiogram of bacterial overlay assay performed on vaginal fluid proteins. Proteins 1, 2, and 3 bind radiolabeled bacteria. Protein 2 in panel A (arrow) resolves as a single spot with an altered molecular mass in panel B (arrow).
