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. 1999 Oct;67(10):5027–5032. doi: 10.1128/iai.67.10.5027-5032.1999

FIG. 4.

FIG. 4

Detection of S-IgA and bacterial overlay assay of S-IgA in vaginal fluid. (A) A 50-μg sample of vaginal fluid was subjected to Western blotting with polyclonal anti-S-IgA antibody. (B) Autoradiograph of bacterial overlay assay performed on 20 μg of S-IgA, using 35S-labeled type 1-piliated E. coli (HB101/pWRS1-17).