Table 1.

List of tentative RBD mutations (month-wise Pan-India) found in GISAID database from January–May 2021 of alpha and delta variant, from December 2021–January 2022 of omicron variant and May 2022–Aug 2022 Omicron sub-variant

Month and Year	No. of sequences deposited	Mutations in the RBD domain
January (2021)	633	No mutation
February (2021)	1209	V367F (alpha), E484K (alpha), N501Y (alpha)
March (2021)	2257	T333R (delta), F338L (alpha), P384S (alpha), G413R (delta), V445A(alpha), G446V(alpha), L452R (Delta), T478K (Delta), V483F(delta), E484K(alpha), N501Y(alpha) and A520S(alpha)
April (2021)	2782	S349Q (delta), S375A (delta), Q414R (delta), A435T (delta), G446S (delta), L452R (Delta), T470S (delta), E471G (delta), S477I (delta), T478K (Delta), V483L (delta), E484K(alpha), C488F (delta), Y495M (delta), N501Y(alpha), Q506K (delta), P507L (delta), E516Q (delta) and A520S (delta)
May (2021)	818	R346G (delta), A348S (delta), A352S (delta), G381A (delta), G413V (delta), G446V (delta) Y451H (delta), G476S (delta), S477I (delta) and F490L (delta)
Dec 2021-Jan 2022	435	G339D, R346K, V367A, S371L, S373P, F374V, S375F, T376A, C379R, D405N, R408S, Q414K, K417N, N440K, G446S, L452R, S477N, T478K, E484A/Q, Q493R, G496S, Q498R, N501Y, Y505H, A520E, G526K, P527K
May 2022-Aug 2022	1856	D339H, R346K, V367A, S371L, S371F, S373P, F374V, S375F, T376A, C379R, D405N, R408S, Q414K, K417N, N440K, G446S, L452R, N460K, S477N, T478K, E484A/Q, R493Q, G496S, Q498R, N501Y, Y505H, A520E, G526K, P527K

Mutations occur between Jan–May, 2021 have selective presence in alpha and delta variants in individual samples