Fig. 5.

Fig. 5. Effect of SCA and SCB on IL-1α secretion in TNFα-stimulated HaCaT cells. HaCaT cells were either treated or un-treated with 10 μM of SCA and SCB or the combined mix of the two compounds at either 10 and 20 μM doses. After 24 h, cells were stimulated with 10 ng/mL TNFα for 1 h. ELISA assay was used to determine IL-1α release in the cell culture supernatants 24 h after the treatments. Values represent mean ± SEM. Data are representative of three independent experiments. Statistical significance determined by two-way ANOVA. *vs control (DMSO); ^#vs TNFα treated samples; p < 0.05