FIG. 2.

Partial restriction map of the H. ducreyi 35000 chromosomal DNA insert in pJKT933 and related plasmids. The two complete ORFs and two incomplete ORFs in this insert are indicated together with arrows designating the proposed direction of transcription. Restriction sites in parentheses indicate vector cloning sites. The open arrows indicate various oligonucleotide primers used in PCR. The cross-hatched bar beneath the znuA gene indicates the 1-kb probe used for Southern blot analysis in Fig. 4. The kan2 cartridge was ligated into the MscI site of pJKT933 to construct pDL5-3. Plasmid pDL6-2 is a modified pCW170 vector (see Materials and Methods) containing the 1.8-kb XhoI- and PvuI-digested PCR product with the znuA gene and flanking DNA.