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. 1999 Oct;67(10):5060–5068. doi: 10.1128/iai.67.10.5060-5068.1999

FIG. 4.

FIG. 4

Southern blot analysis of chromosomal DNA preparations from H. ducreyi wild-type and mutant strains. Chromosomal DNAs were digested with SpeI, resolved by agarose gel electrophoresis, and probed with either a 1-kb SpeI fragment derived from the H. ducreyi znuA gene (lanes 1 and 2) or with the kan2 cartridge (lanes 3 and 4). Lanes 1 and 3, strain 35000; lanes 2 and 4, znuA mutant 35000.901. Size markers (in kilobases) are on the left side of the figure.