FIG. 5.

Growth of wild-type, mutant, and recombinant strains of H. ducreyi and H. influenzae in vitro. These data are from representative experiments. (A) H. ducreyi strains grown overnight on CA-Zn plates were inoculated into broth with (solid symbols) and without (open symbols) 100 μM ZnCl₂, and growth was monitored for 36 h. Wild-type strain 35000 is indicated by squares, and znuA mutant 35000.901 is indicated by circles. (B) H. ducreyi strains grown overnight on CA-Zn plates were inoculated into broth without supplemental zinc, and growth was monitored for 32 h. Symbols: squares, wild-type H. ducreyi 35000; circles, H. ducreyi znuA mutant 35000.901; diamonds, H. ducreyi znuA mutant 35000.901(pCW170); triangles, H. ducreyi znuA mutant 35000.901(pDL6-2). (C) H. influenzae strains grown overnight on BHIs plates containing 100 μM ZnCl₂ were inoculated into BHIs broth without supplemental zinc, and growth was monitored for 14 h. Symbols: squares, wild-type NTHI 6564; circles, NTHI 6564 pzp1 mutant; diamonds, NTHI 6564 pzp1 mutant containing pCW170; triangles, NTHI 6564 pzp1 mutant containing pDL6-2.