TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Reference or source |
|---|---|---|
| E. coli | ||
| DH5α | Host strain for cloning experiments | 54 |
| HB101 | Host strain essential for propagating plasmids carrying mutated H. ducreyi DNA inserts used in electroporation | 6, 54 |
| XL1-Blue | Host strain used for screening of the ZAP Express bacteriophage library | Stratagene |
| XLOLR | Host strain used for excision of recombinant pBK-CMV plasmids from the ZAP Express bacteriophage | Stratagene |
| H. influenzae | ||
| NTHI 6564 | Wild-type parent strain | 39 |
| NTHI 6564 pzp1 mutant | pzp1 mutant of NTHI 6564 | 39 |
| NTHI 6564.pzp1(pCW170) | NTHI 6564 pzp1 mutant containing the vector pCW170 | This study |
| NTHI 6564.pzp1(pDL6-2) | NTHI 6564 pzp1 mutant containing pDL6-2 with the H. ducreyi znuA gene | This study |
| H. ducreyi | ||
| 35000 | Wild-type strain isolated in Winnipeg, Manitoba, Canada | 29 |
| 35000.901 | Isogenic mutant with a kan2 cartridge inserted into the MscI site in the znuA gene | This study |
| 35000.901(pCW170) | 35000.901 containing the vector pCW170 | This study |
| 35000.901(pDL6-2) | 35000.901 containing pDL6-2 with the H. ducreyi znuA gene | This study |
| RO18 | Wild-type strain from Kenya | 3 |
| Cha-1 | Wild-type strain from Dallas, Tex. | 31 |
| A77 | Type strain from Institut Pasteur | 44 |
| 1151 | Wild-type strain from the Gambia | 57 |
| 1153 | Wild-type strain from the Gambia | Allan Ronald |
| 511 | Wild-type strain from Thailand | Allan Ronald |
| 512 | Wild-type strain from Thailand | 57 |
| Hd12 | Wild-type strain from Korea | 43 |
| 1352 | Wild-type strain from Kenya | 57 |
| 78226 | Wild-type strain from Winnipeg, Manitoba, Canada | 3 |
| 6V | Wild-type strain from Atlanta, Ga. | 3 |
| BG411 | Wild-type strain from Kenya | 3 |
| O41 | Wild-type strain from Sweden | 57 |
| 1145 | Wild-type strain from Amsterdam | 57 |
| Plasmids | ||
| pUC18 | Cloning vector, Ampr | 54 |
| pUC18K2 | pUC18 with the kan2 cartridge containing the promoterless aphA-3 gene | James Kaper |
| pBK-CMV | Phagemid excised from the ZAP Express cloning vector | Stratagene |
| pJKT913 | pBK-CMV with a 3.7-kb H. ducreyi DNA insert containing the znuA gene | This study |
| pBluescript II KS(−) | Cloning vector, Amp | Stratagene |
| pJKT933 | pBluescript II with a 3.7-kb HindIII-SacI fragment from pJKT913 containing the znuA gene | This study |
| pDL5-3 | pJKT933 with a kan2 cartridge inserted into the MscI site within the H. ducreyi znuA ORF | This study |
| pLS88 | Cloning vector capable of replication in H. ducreyi; Kanr Smr Sulr | 19 |
| pCW170 | pLS88 with a cat cartridge inserted into the EcoRI site of this vector | This study |
| pDL6-2 | pCW170 with a 402 nt PvuI-XhoI fragment removed from the kan gene and a 1.8-kb PCR-derived, XhoI-PvuI DNA fragment with the H. ducreyi znuA gene inserted in its place | This study |