Table 2.

List of different gene targets and primers used in lab-based and point-of-care nucleic acid amplification tests for SARS-CoV-2 around the world.

Institute	Gene target	Probe (5′-3′)	Forward primer (5′-3′)	Reverse primer (5′-3′)
China CDC	ORF1ab gene	FAM-CCGTCTGCGGTATGT GGAAAGGTTATGG-BHQ1	CCCTGTGGGTTTTACACTTAA	ACGATTGTGCATCAGCTGA
	N gene	FAM-TTGCTGCTGCTTGACAGA TT-TAMRA	GGGGAACTTCTCCTGCTAGAAT	CAGACATTTTGCTCTCAAGCTG
US CDC	N1 target	FAM-ACCCCGCATTAC GTT TGGTGGACC-BHQ1	GAC CCC AAA ATC AGC GAA AT	TCT GGT TAC TGC CAG TTG AAT CTG
	N2 target	FAM-ACAATTTGCCCCCAGCGC TTCAG-BHQ1	TTA CAA ACA TTG GCC GCA AA	GCG CGA CAT TCC GAA GAA'
	N3 target	FAM-AYCACATTGGCACCCGCA ATCCTG-BHQ1	GGG AGC CTT GAA TAC ACC AAA A	TGT AGC ACG ATT GCA GCA TTG
France Pasteur Institute	RdRP1 target	HEX-AGATGTCTTGTGCTGCCG GTA-BHQ1	ATGAGCTTAGTCCTGTTG	CTCCCTTTGTTGTGTTGT
	RdRP2 target	FAM-TCATACAAACCACGCCAG G-BHQ1	GGTAACTGGTATGATTTCG	CTGGTCAAGGTTAATATAGG
Japan National Institute of Infectious Disease.	N gene	FAM-ATGTCGCGCATTGGCATG GA-BHQ	AAATTTTGGGGACCAGGAAC	TGGCAGCTGTGTAGGTCAAC
Germany Charité	RdRP gene	FAM-CAGGTGGAACCTCATCAG GAGATGC-BBQ	GTGARATGGTCATGTGTGGCGG	CARATGTTAAASACACTATTAGCATA
	E gene	FAM-ACACTAGCCATCCTTACTGCGCTTCG-BBQ	ACAGGTACGTTAATAGTTAATAGCGT	ATATTGCAGCAGTACGCACACA
Thailand National Institute of Health	N gene	FAM-CAACTGGCAGTAACCA-BQH1	CGTTTGGTGGACCCTCAGAT-	CCCCACTGCGTTCTCCATT
Hong Kong University	ORF1b-nsp14 gene	FAM-TAGTTGTGATGCWATCATGACTAG-TAMRA	TGGGGYTTTACRGGTAACCT	AACRCGCTTAACAAAGCACTC
	N gene	FAM-GCAAATTGTGCAATTTGCGG-TAMRA	TAATCAGACAAGGAACTGATTA	CGAAGGTGTGACTTCCATG