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Production of IL-8 by HEp-2 cell requires M. avium uptake. Cells were treated or not treated with cytochalasin D (CD) for 30 min (known to prevent M. avium uptake) and then exposed to M. avium 101 (MAC101) (MOI 10) after 2 h; extracellular bacteria were removed by washing. Supernatants were obtained after 24, 48, and 72 h, and the IL-8 concentration was determined. Production of IL-8 was not observed on cytochalasin D-treated monolayers.
