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Dooku1 inhibits Yoda1-induced increase in HAoSMCs calcification. (A,B) HAoSMCs were exposed to OM (OM; excess Pi: 1.5 mmol/L, excess calcium: 0.6 mmol/L) in the presence or absence of Yoda1 (10 μmol/L) and Dooku1 (1 or 5 μmol/L). (A) Representative Alizarin Red staining (day 6) and quantification. (B) Calcium content of HCl-solubilized ECM (day 6). Data are presented as mean ± SD, n = 3–5. *p < 0.05, ***p < 0.005, ****p < 0.001.
