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Piezo1 is critical in OM-induced HAoSMCs calcification. (A,B) HAoSMCs were exposed to control medium (GM) or OM (excess Pi: 1.5 mmol/L, excess calcium: 0.6 mmol/L) in the presence of Dooku (5 μmol/L). (A) Representative Alizarin Red staining (day 6) and quantification. (B) Calcium content of HCl-solubilized ECM (day 6). Data are presented as mean ± SD, n = 4–5. **p < 0.01, ****p < 0.001.
