Figure 4.

Nanoneedle interfacing with the explanted human corneal endothelium. A) Schematic representation of the nanoinjection approach for explanted human corneas. Image created with Biorender.com. B,C) Immunofluorescence confocal microscopy of the interface between nanoneedles and the endothelium of human cornea explants. Images were obtained immediately after nanoneedle assisted interfacing by centrifugation. Nanoneedles colocalized with HCEnCs and did not protrude beyond them. ZO‐1 (red) localizes in HCEnCs membrane, FITC (green) labels nN and DAPI (blue) nuclear counterstain. B) 3D reconstruction from Z‐stack. C) Orthogonal projections. Scale bar 20 µm.