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. 2022 Oct 17;9(33):2203257. doi: 10.1002/advs.202203257

Figure 5.

Figure 5

Nanoneedle interfacing preserves cornea integrity. A) Immunofluorescence confocal microscopy image of the explanted corneal endothelium obtained 72 h after nanoneedles interfacing, showing a maintained native endothelial morphology. ZO‐1 (green) staining with DAPI (blue) nuclear counterstain. Scale bar 20 µm. B) Immunofluorescence microscopy of Caspase 3/7 activation 72 h after nanoneedles interfacing. Lack of nuclear staining with faint cytoplasmic staining, comparable to untreated control demonstrate lack of Caspase 3/7 activation, indicating absence of apoptotic events. Caspase 3/7 (green) staining with DAPI (blue) nuclear counterstain. Scale bar 20 µm. C) Fluorescence microscopy of ctr and nN treated corneas (10 µm OCT sections) stained with DAPI (blue) shows that corneal thickness was unchanged in either of the two conditions. Scale bar 100 µm. D) Representative images of explanted corneas before and after (72 h) nanoinjection, showing how corneal clarity is maintained. E) Fluorescence microscopy of nN treated corneal sections, showing the expected expression and localization of epithelial (EPI, p63, and CK12) and endothelial (ENDO, CK18) cell markers (green). DAPI (blue) counterstains nuclei. Scale bar 50 µm.