Skip to main content
. 2022 Oct 17;9(33):2203257. doi: 10.1002/advs.202203257

Figure 6.

Figure 6

Effects of p16‐siRNA nanoinjection to the explanted human corneal endothelium. A) Immunofluorescence microscopy of siGlo nanoinjection to the endothelium of explanted human corneas. Images were obtained 48 h after nanoinjection. HCEnCs cells display cytosolic siGlO in the area of nanoinjection (nN) as compared to untreated controls (ctr). siGlo signal (red) with DAPI (blue) nuclear counterstain. Scale bar 20 µm. B) Immunofluorescence microscopy of p16 protein expression in NSC and siRNA treated HCEnCs of explanted corneas. p16 (green) staining with DAPI (blue) nuclear counterstain. Scale bar 50 µm. C) Immunofluorescence microscopy of explanted human corneas 72 h following nanoinjection of p16 siRNA (n = 3). A significant correlation is visible between siGlo signal and loss of p16 signal, as highlighted by the white asterisks. p16 (green), siGlo (red) staining with DAPI (blue) nuclear counterstain. Scale bar 50 µm. D) Immunofluorescence quantification evaluating the fraction of p16 negative cells in siGlo+ transfected and siGlo‐ untransfected HCEnCs. Values are represented as mean + SD. Two‐sided t‐test was used to assess statistical significance, p = 1.5e‐8. E) Immunofluorescence quantification of p16 expression levels in siGlo+ transfected and siGlo‐untransfected cells. Values are represented as mean + SD. Two‐sided t‐test was used to assess statistical significance, p = 0.0002.