TABLE 1.
Cell number and viability of HeLa cells treated with C. botulinum C2 toxina
| Cells | No. of cells/dish (dead cells as % of total cell no.) after incubation for:
|
||
|---|---|---|---|
| 24 h | 48 h | 72 h | |
| Control | 1.7 × 105 (<10) | 3.2 × 105 (<10) | 6.0 × 105 (<10) |
| C2 treated | 1.0 × 105 (<10) | 1.0 × 105 (<10) | 1.2 × 105 (30) |
a
HeLa cells were grown in complete medium at 37°C in the absence (control) or in the presence of C2 toxin (200 ng of C2II and 100 ng of C2I per ml). After 24, 48, and 72 h, the cells were incubated for 30 min with 0.36% trypan blue in complete medium, and the total cell number and the number of cells unable to exclude trypan blue were determined with a Neubauer chamber.