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. 2022 Nov 10;13:979368. doi: 10.3389/fmicb.2022.979368

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Copyright © 2022 Kong, Feng, Zhao, Chen, Wang, Chen, Shao, Liao, Li, Xie, Zhang and Xie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Replicative capacity of live attenuated duck enteritis virus (DEV) vaccine to chicken embryos in vivo. (A) Pathogenicity and gross damage of live attenuated DEV vaccine in chicken embryos. (B) Virus replication of live attenuated DEV vaccine in chicken embryos. SPF-chicken embryos, 1-day-old, were randomly divided into four groups, and each group covers ten chicken embryos. From left to right being challenged with DEV AV1221 (10⁵.⁴² TCID₅₀), 1 dose of vaccine (10³.⁰⁰ ELD₅₀), and 10 dose of vaccine (10⁴.⁰⁰ ELD₅₀), at the same dose in 100 μL, respectively. The results are representative of two tests. ^****P < 0.0001.