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. 2022 Nov 10;13:979368. doi: 10.3389/fmicb.2022.979368

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Copyright © 2022 Kong, Feng, Zhao, Chen, Wang, Chen, Shao, Liao, Li, Xie, Zhang and Xie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Live attenuated duck enteritis virus (DEV) vaccine replicated in non-target chickens and resulted in effectively virus transmission. (A) Pathogenic lesions of non-target chickens. (B) The hematoxylin- and eosin (H&E)-stained tissue section in non-target chickens. From left to right are liver, heart, spleen, glandular stomach, liver, kidney, and intestine. (C) The survival curve of non-target chickens. (D) DEV effectively replicates in different tissue of non-target chickens. Data are representative of four experiments related to challenge. ^**P < 0.01, ^***P < 0.001, and ^****P < 0.0001.