Table 5.

Organic acid distribution for shake flask cultivations of A. oryzae with different initial product concentrations

Conditiona	Organic acid distribution [%]b
	Malate	Succinate	Fumarate	Pyruvate	α-Ketoglutarate	Oxalate	Citrate
2 g/L M	50.1 ± 1.3	40.0 ± 2.7	6.1 ± 0.5	0.9 ± 0.1	0.3 ± 0.0	1.7 ± 0.5	0.9 ± 0.4
6 g/L M	46.7 ± 0.9	38.4 ± 1.9	10.8 ± 0.4	0.8 ± 0.0	0.5 ± 0.1	1.9 ± 0.5	1.0 ± 0.5
10 g/L M	42.3 ± 1.6	35.3 ± 1.5	15.6 ± 0.5	0.9 ± 0.1	0.5 ± 0.1	3.9 ± 0.7	1.5 ± 0.8
20 g/L M	36.0 ± 3.4	34.6 ± 2.6	23.3 ± 3.6	1.0 ± 0.2	0.4 ± 0.1	3.7 ± 2.2	1.0 ± 0.3
35 g/L M	26.9 ± 1.6	35.3 ± 1.9	34.1 ± 1.7	1.0 ± 0.1	0.3 ± 0.0	2.0 ± 0.7	0.3 ± 0.3
50 g/L M	16.6 ± 3.0	35.1 ± 1.6	45.5 ± 4.2	0.9 ± 0.1	0.3 ± 0.0	1.6 ± 0.5	0.0 ± 0.0
1 + 1 g/L M + S	52.2 ± 1.6	40.2 ± 3.0	4.6 ± 0.3	0.7 ± 0.0	0.3 ± 0.1	1.5 ± 0.8	0.4 ± 0.2
3 + 3 g/L M + S	51.9 ± 0.6	34.3 ± 2.4	8.3 ± 0.5	0.8 ± 0.2	0.4 ± 0.1	3.3 ± 2.3	1.0 ± 0.1
5 + 5 g/L M + S	50.3 ± 2.6	34.7 ± 6.8	10.3 ± 1.2	0.9 ± 0.1	0.5 ± 0.1	2.8 ± 2.7	0.7 ± 0.4
10 + 10 g/L M + S	44.0 ± 0.5	40.0 ± 0.6	12.3 ± 0.1	0.8 ± 0.1	0.4 ± 0.0	1.5 ± 0.3	1.0 ± 0.1
17.5 + 17.5 g/L M + S	37.8 ± 0.8	38.9 ± 2.0	18.3 ± 0.7	0.8 ± 0.1	0.3 ± 0.0	3.2 ± 2.4	0.7 ± 0.1
25 + 25 g/L M + S	32.9 ± 1.6	39.6 ± 0.9	23.1 ± 1.5	0.8 ± 0.1	0.3 ± 0.0	2.7 ± 0.7	0.5 ± 0.1
Control	53.1 ± 0.5	37.4 ± 0.6	4.0 ± 0.1	0.7 ± 0.0	0.5 ± 0.0	2.8 ± 1.0	1.4 ± 0.2

^aM  malic acid, S  succinic acid

^bValues refer to the organic acid distribution measured at 240 h minus the initial concentrations of malic and succinic acid and are given as percentage of the total acid concentration which is displayed in Table 4

Data represent means ± standard deviation, n = 3