Fig. 6.

Keap1 activation and Nrf2/Bach1 pathway inhibition are required for the UBR7-induced HK2 and glycolysis suppression. A The levels of glycolysis, glucose uptake, pyruvate and lactate secretion in UBR7-KO cells overexpressing Keap1. B The levels of glycolysis, glucose uptake, and pyruvate and lactate secretion in BEL-7402 cells overexpressed UBR7 and knocked out Keap1. C The levels of glycolysis, glucose uptake, pyruvate and lactate secretion in UBR7-KO cells knocked out Nrf2. D The levels of glycolysis, glucose uptake, pyruvate and lactate secretion in UBR7-KO cells treated with Hemin. E The levels of colony number in UBR7-KO cells treated with Hemin. F UBR7-KO cells treated with Hemin were injected subcutaneously into nude mice to analyse xenogeneic tumours, tumour weight, lymph node metastasis and lymph node size levels. Data are shown as mean ± SD of three independent experiments. **p < 0.01. The scale bar in (E) was 100 μm