Fig. 5.

Relative expression of the folate transport systems in brain parenchymal cells. A Relative mRNA expression of mouse Slc19a1 (RFC), Slc46a1 (PCFT), and Folr1 (FRα) was assessed in primary cultures of mouse neurons, and primary cultures of mouse mixed glial cells. Results are presented as mean relative mRNA expression normalized to the housekeeping gene mouse cyclophilin B (n = 4). B–D Representative immunoblots from three separate experiments demonstrating protein expression of RFC, PCFT, and FRα in primary cultures of mouse neurons, and in primary cultures of mixed glial cells. HEK293 and HepG2 cells were used as positive controls for the three transport systems, with mouse kidney used as an additional positive control for FRα. Multiple protein bands for RFC and PCFT represent differential glycosylation