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Assessing cytotoxicity of ND-TSL and free ND at various concentrations (12.5–36.5 μg/mL) utilizing MTT assay and MDA-MB-231 cells at (A) 37 °C and (B) 40 °C for 24 h. An overall statistically significant decrease in cell viability was observed with ND-TSL compared to ND. Untreated cells were used as negative control and considered as 100%. All experiments were carried out in triplicate, and the mean values were calculated. Error bars represent ± standard deviation.
