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. 2022 Nov 10;13:994496. doi: 10.3389/fimmu.2022.994496

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Copyright © 2022 Pérez-Figueroa, Álvarez-Carrasco and Ortega

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Myeloperoxidase and Neutrophil elastase are associated with extracellular DNA released after CD13 crosslinking. Confocal microscopy images of human neutrophils stimulated for 240 min with mAb 452, or Fab 452 plus F(ab’)2 fragments of anti-mouse Ig, or left unstimulated (control). Cells were fixed and stained to visualize DNA (blue), myeloperoxidase (red) or neutrophil elastase (green). Each immunofluorescence image is shown together with its respective bright field micrograph. Inserts show an enlargement of a selected area of each image. Bars: 50 µm.