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. 2022 Oct 23;11(11):2089. doi: 10.3390/antiox11112089

Figure 4.

Figure 4

Treatment with the GSH inhibitor butthionine sulfoximine (BSO) reversed the ability of narcissoside (NCS) to inhibit 6-OHDA-induced ROS production and expression of apoptosis-related proteins in SH-SY5Y cells. BSO (100 μM) or NAC (1 mM) was added to SH-SY5Y cells 1 h before NCS pretreatment. Then, cells were incubated with NCS (1 μM) for 24 h, followed by treating them with 100 μM 6-hydroxydopamine (6-OHDA) for 12 h. (A) ROS levels were measured using the H2DCFDA method. (B) GSH levels were determined by GSH assay kit. (C) The ratio of cleaved caspase 3/pro caspase 3 and cleaved PARP/pro PARP was determined by western blotting. The expression of β-tubulin is internal loading control (left). The intensity of the signal was quantified by the ImageJ software. (right).