Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Oct 23;11(11):2089. doi: 10.3390/antiox11112089

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Downregulation of Nrf2 expression in the 6-OHDA-exposed SH-SY5Y cells abrogates the neuroprotective ability of narcissoside (NCS). SH-SY5Y cells were transfected with Nrf2 siRNA or control siRNA for 24 h. It was then pretreated with NCS for 24 h, followed by treatment with 6-OHDA for 6 h. (A) The levels of Nrf2, GCLC, and GCLM were analyzed by western blotting. The expression of β-tubulin is internal loading control (top left). The intensity of the signal was quantified by the ImageJ software (right and bottom). (B) ROS levels were measured using the H2DCFDA method. (C) GSH levels were determined by GSH assay kit. (D) Protein levels of JNK, p-JNK, p38, p-p38, ERK1/2, p-ERK1/2, Akt and p-Akt were determined by western blot. The expression of β-tubulin is as internal loading control. (top left). The intensity of the signal was quantified by the ImageJ software. (right and bottom). (E) The ratio of cleaved caspase 3/pro caspase 3 and cleaved PARP/pro PARP was determined by western blotting. The expression of β-tubulin is internal loading control (top left). The intensity of the signal was quantified by the ImageJ software. (right and bottom). (F) The ratio of apoptotic cells was determined by TUNEL assay.

Downregulation of Nrf2 expression in the 6-OHDA-exposed SH-SY5Y cells abrogates the neuroprotective ability of narcissoside (NCS). SH-SY5Y cells were transfected with Nrf2 siRNA or control siRNA for 24 h. It was then pretreated with NCS for 24 h, followed by treatment with 6-OHDA for 6 h. (A) The levels of Nrf2, GCLC, and GCLM were analyzed by western blotting. The expression of β-tubulin is internal loading control (top left). The intensity of the signal was quantified by the ImageJ software (right and bottom). (B) ROS levels were measured using the H2DCFDA method. (C) GSH levels were determined by GSH assay kit. (D) Protein levels of JNK, p-JNK, p38, p-p38, ERK1/2, p-ERK1/2, Akt and p-Akt were determined by western blot. The expression of β-tubulin is as internal loading control. (top left). The intensity of the signal was quantified by the ImageJ software. (right and bottom). (E) The ratio of cleaved caspase 3/pro caspase 3 and cleaved PARP/pro PARP was determined by western blotting. The expression of β-tubulin is internal loading control (top left). The intensity of the signal was quantified by the ImageJ software. (right and bottom). (F) The ratio of apoptotic cells was determined by TUNEL assay.