Table 3.
Gradient elution conditions of the HPLC-DAD analyses for the identification and quantification of polyphenolic compounds.
Time (Min.) | Composition A% (Water+Formic Acid 0.1%) | Flow (mL/min) |
---|---|---|
1 | 97 | 0.6 |
5 | 77 | 0.6 |
12 | 73 | 0.6 |
18 | 57 | 0.6 |
25 | 52 | 0.6 |
32 | 50 | 0.6 |
34 | 50 | 0.6 |
37 | 35 | 0.6 |
40 | 5 | 0.6 |
47 | 5 | 0.6 |
48 | 97 | 0.6 |
60 | 97 | 0.6 |