Table 2.
Antimicrobial activity of the crocCATHs. The MIC values of the synthetic peptides were determined in a microdilution assay in MHB. The antibiotic vancomycin was used against Gram-positive S. aureus and E. faecium whereas polymyxin B was employed against the remaining Gram-negative strains. Values in μg/mL can be found in Supplementary Table S4.
| Bacteria | MIC in μM | |||||
|---|---|---|---|---|---|---|
| As-CATH7 | As-CATH8 | Gg-CATH5 | Gg-CATH7 | Polymyxin B | Vancomycin | |
| E. cloacae | 0.5 | 0.5 | 0.5 | 2 | 0.5 | n.d. |
| S. aureus | 4 | 0.5 | 1 | 16 | n.d. | 0.5 |
| K. pneumoniae | 1 | 0.5 | 0.5 | 4 | 0.5 | n.d. |
| A. baumannii | 0.25 | 0.25 | 0.5 | 1 | 0.5 | n.d. |
| P. aeruginosa | 4 | 1 | 1 | 8 | 0.5 | n.d. |
| E. faecium | >64 | >64 | >64 | 64 | n.d. | >64 |
| E. coli | 2 | 1 | 4 | 4 | 0.5 | n.d. |
| S. Typhimurium | 2 | 1 | 0.5 | 4 | 1 | n.d. |
| P. vulgaris | >64 | 4 | 8 | >64 | >64 | n.d. |
n.d.: not determined.