FIG. 3.

Antibody titers from mice immunized with the pcdna3 SERA 17-382 DNA vaccine. Mice were immunized by i.m. injection or Gene Gun, as indicated. Serum was collected at weeks 6 and 8; all mice were immunized at weeks 0 and 4. Serial dilutions were used to measure the end point-positive titers. The results presented are the averages of two separate ELISAs. The titer of each serum sample is the inverse of the largest dilution at which the substrate colorometric development is above that of a negative control. The variation in the end point-positive titers was always within one “twofold serial dilution.” Preimmune serum titers for the SERA protein were <80 in this assay.