Table 1.
Ingredients and chemical composition of experimental diets fed to laying hens.
| Treatments 1: Amaranth × Enzyme | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Item | T1: 0 × 0 |
T2: 100 × 0 |
T3: 200 × 0 |
T4: 300 × 0 |
T5: 400 × 0 |
T6: 0 × 0.25 |
T7: 100 × 0.25 |
T8: 200 × 0.25 |
T9: 300 × 0.25 |
T10: 400 × 0.25 |
| Ingredient, (g/kg) | ||||||||||
| Corn | 614.9 | 548.0 | 481.5 | 415.7 | 326.9 | 612.2 | 545.4 | 478.9 | 413.0 | 329.4 |
| Soybean meal | 235.6 | 217.0 | 198.2 | 178.6 | 162.0 | 236.5 | 217.8 | 199.1 | 179.5 | 162.0 |
| Amaranth grain | 0.0 | 100.0 | 200.0 | 300.0 | 400.0 | 0.0 | 100.0 | 200.0 | 300.0 | 400.0 |
| Oyster shell | 96.7 | 91.1 | 85.4 | 79.7 | 85.5 | 96.7 | 91.1 | 85.4 | 79.7 | 83.0 |
| Vegetable oil | 24.0 | 16.2 | 08.4 | 00.3 | 00.0 | 25.8 | 18.0 | 10.1 | 02.1 | 00.0 |
| Dicalcium phosphate | 18.8 | 18.8 | 18.8 | 18.7 | 18.6 | 18.8 | 18.8 | 18.8 | 18.7 | 18.6 |
| Vitamin premix 2 | 2.5 | 2.5 | 2.5 | 2.5 | 2.5 | 2.5 | 2.5 | 2.5 | 2.5 | 2.5 |
| Mineral premix 3 | 2.5 | 2.5 | 2.5 | 2.5 | 2.5 | 2.5 | 2.5 | 2.5 | 2.5 | 2.5 |
| Common salt | 2.0 | 2.0 | 2.0 | 2.0 | 2.0 | 2.0 | 2.0 | 2.0 | 2.0 | 2.0 |
| DL-methionine | 3.0 | 1.9 | 0.7 | 0.0 | 0.0 | 3.0 | 1.9 | 0.7 | 0.0 | 0.0 |
| Natuzyme P50 enzyme | 00.0 | 00.0 | 00.0 | 00.0 | 00.0 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 |
| Calculated nutrient content, (g/kg) | ||||||||||
| AMEn (Mj/kg) | 11.84 | 11.84 | 11.84 | 11.84 | 11.84 | 11.84 | 11.84 | 11.84 | 11.84 | 11.84 |
| Crude protein (g/kg) | 152.5 | 152.5 | 152.5 | 152.5 | 152.5 | 152.5 | 152.5 | 152.5 | 152.5 | 152.5 |
| Ether extract (g/kg) | 50.2 | 46.4 | 42.5 | 38.5 | 41.4 | 51.8 | 48.0 | 44.1 | 38.7 | 41.4 |
| Crude fiber (g/kg) | 27.8 | 38.3 | 48.8 | 59.2 | 69.4 | 27.8 | 38.2 | 48.7 | 59.2 | 69.4 |
| Linoleic acid (g/kg) | 15.8 | 39.5 | 43.1 | 50.6 | 58.6 | 15.8 | 39.5 | 43.1 | 50.6 | 58.6 |
| Calcium (g/kg) | 43.5 | 43.5 | 43.5 | 43.5 | 43.5 | 43.5 | 43.5 | 43.5 | 43.5 | 43.5 |
| Available phosphorus (g/kg) | 4.6 | 4.6 | 4.6 | 4.6 | 4.6 | 4.6 | 4.6 | 4.6 | 4.6 | 4.6 |
| Methionine (g/kg) | 3.7 | 4.1 | 4.2 | 4.2 | 4.2 | 3.7 | 4.1 | 4.2 | 4.2 | 4.2 |
| Met + cysteine (g/kg) | 6.7 | 6.7 | 6.7 | 7.2 | 7.2 | 6.7 | 6.7 | 6.7 | 7.2 | 7.2 |
| Lysine (g/kg) | 0.78 | 0.79 | 0.81 | 0.81 | 0.82 | 0.78 | 0.79 | 0.81 | 0.81 | 0.82 |
| Arginine (g/kg) | 8.1 | 8.2 | 8.2 | 8.5 | 8.6 | 8.1 | 8.2 | 8.2 | 8.5 | 8.6 |
| Threonine (g/kg) | 5.7 | 5.8 | 5.9 | 6.1 | 6.1 | 5.7 | 5.8 | 5.9 | 6.1 | 6.1 |
| DCAB (mEq/kg) | 201.47 | 201.47 | 201.47 | 201.47 | 201.47 | 201.47 | 201.47 | 201.47 | 201.47 | 201.47 |
1Treatments (g/kg): T1: 0 AG(−E), T2: 100 AG(−E), T3: 200 AG(−E), T4: 300 AG(−E), T5: 400 AG(−E), T6: 0 AG(+E), T7: 100 AG(+E), T8: 200 AG(+E), T9: 300 AG(+E), T10: 400 AG(+E); 2 vitamin supplement provided per kilogram of diet:vitamin A, 8000 IU; vitamin E, 20 IU; menadione, 3.0 mg; vitamin D3, 2000 IU; riboflavin, 4.0 mg; Ca-pantothenate, 12 mg; nicotinic acid, 50 mg; choline, 300 mg; vitamin B12, 15 mg; vitamin B6, 0.12 mg; thiamine, 1.5 mg; folic acid, 1.00 mg; D-biotin, 0.10 mg; 3 mineral supplement provided per kilogram of diet: trace mineral (milligrams per kilogram of diet): Mn, 100; Zn, 70; Fe, 50; Cu, 10; iodine, 1; Se, 0.30; antioxidant, 50.